PCR for IBV, IBD, NDV

Test code: PCR-020

Category:
Birds
Sub Category:
Poultry
Price (PKR) 21,000 Request a test
TypeRT-PCR (Reverse Transcription PCR)
MethodPCR
StatusActive
Test DescriptionPCR
Test Days0
Test Turnaround (business days)1

Cloacal Swabs

Preferred
SpeciesPoultry and other avian species
Sample DetailsCloacal swabs collect material from the common terminal opening of the digestive, urinary, and reproductive tracts, making them highly informative for detection of enteric and systemic pathogens, particularly in poultry. They are especially useful for identifying actively shedding or carrier birds. Submission of cloacal swabs is recommended in cases of: surveillance for enteric or zoonotic pathogens, detection of carrier status in apparently healthy birds, monitoring infection spread within flocks, post-vaccination or biosecurity screening, laboratory analysis of cloacal swabs supports early pathogen detection, assessment of shedding dynamics, and implementation of effective disease control and food safety measures.
Ideal Volume10 Swabs
Minimum Volume1 Swab
Sample ContainerSterile swab with appropriate transport medium, Leak Proof
Storage & Transport2–8 °C,
Rejection CriteriaDry swabs. Broken or leaking container. Improper labeling. Contamination. Delayed transport. Improper storage.

Tracheal, Oropharyngeal Swabs

SpeciesPoultry
Sample DetailsTracheal and oropharyngeal swabs sample the upper and mid-respiratory tract, where many respiratory pathogens actively replicate and are shed. These sites are critical for early detection of viral and bacterial respiratory infections, especially during the initial phase of disease. Submission of tracheal or oropharyngeal swabs is recommended in cases of: respiratory distress, coughing, or gasping, suspected viral or bacterial respiratory disease, early outbreak investigations, monitoring of respiratory pathogen circulation in flocks or herds, laboratory analysis of these swabs enables rapid and sensitive pathogen detection, confirmation of disease etiology, and timely implementation of control, vaccination, and biosecurity measures.
Ideal Volume10 Swabs
Minimum Volume1 Swab
Sample ContainerSterile, Leak-proof screw cap tube
Storage & Transport2-8 ℃
Rejection CriteriaDry Swabs or without transport medium (PBS). Leaking or Broken Container. Samples at Room Temperature > 24 Hours. Insufficient Volume. Unlabeled Samples.

Air Sacs or Lungs

SpeciesPoultry and other avian species
Sample DetailsAir sacs and lungs are important respiratory tissues for detecting bacterial, viral, and mixed respiratory infections. Their examination is recommended in cases of respiratory distress, increased mortality, air sacculitis, pneumonia, chronic respiratory disease, and respiratory disease outbreaks, supporting accurate diagnosis, assessment of disease severity, and effective treatment, vaccination, and prevention strategies. ایئر سیکس اور پھیپھڑے سانس کی بیماریوں سے متعلق جراثیم کی تشخیص کے لیے اہم بافتے ہیں۔ ان کا معائنہ سانس لینے میں دشواری، بڑھتی ہوئی اموات، ایئر سیکیولائٹس ، نمونیا، دائمی تنفسی بیماریوں، اور تنفسی بیماریوں کے پھیلاؤ کی تحقیقات کے لیے تجویز کیا جاتا ہے۔ یہ نمونے درست تشخیص، بیماری کی شدت کے جائزے، اور مؤثر علاج، ویکسینیشن، اور روک تھام کی حکمتِ عملیوں میں مدد فراہم کرتے ہیں۔
Ideal Volume1-2 ml Tissue Homogenate/ 1-2 Sterile Swab
Minimum Volume1 ml Tissue Homogenate/ 1 Sterile Swab
Sample ContainerSterile leak-proof container or transport medium tube
Storage & Transport2–8 °C
Rejection Criteria1) Autolyzed or decomposed tissue 2) Dry swabs or swabs without transport medium 3) Excessively contaminated samples 4) Insufficient sample quantity 5) Formalin-fixed or chemically preserved tissue خود تحلیل شدہ یا گل سڑ چکے بافتی نمونے خشک سواب یا ٹرانسپورٹ میڈیم کے بغیر جمع کروائے گئے سواب حد سے زیادہ آلودہ نمونے ناکافی مقدار میں جمع کروائے گئے نمونے فارملین میں محفوظ کیے گئے یا کیمیائی طور پر محفوظ شدہ بافتی نمونے